Has anybody worked on standardization of tiamulin using HPLC method and have they encountered any problems related to the stability of the compound?

09 July 2021 1 4K Report

Standardization of tiamulin was tried using HPLC, mobile phase consisting of acetonitrile and 1 percent ammonium carbonate... The rt was detected at 8.3 based on the gradience, however rt kept changing on subsequent attempts... Could it be due to deterioration of the drug being used?

Safdar Bashir

variation in the second decimal place of the retention time, such as ±0.02–0.05 min is normal. Can you specify the range in rt change. Furthermore, following article can help you understand the reasons.

https://www.chromatographyonline.com/view/how-much-retention-time-variation-normal-0

Good luck.

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Science method

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