I want to perform antioxidant assay using DPPH solution. Is it fine if the wax solution is a bit turbid for DPPH assay. Since, turbid solution does not work for antimicrobial assay for me.
Try DMSO, and make a high concentration. Finally, dilute it with water and perform the assay. Always put a same dilution DMSO as control to eliminate the chances of false positive result. Hope that works.
Regards
Bis
Hi Priyanka,
You could try to dissolve your extract in DMSO and use one DMSO control in your anti-microbial study. I also would suggest to check extract solubility in different percentage/ratio of DMSO in saline.
Instead of DMSO, you also can use Polyethylene glycol (PEG) or Tween-20.
Hope for the best.
For antioxidant assays, find the attached of this review paper.
Thank you Biswajit Mishra, Md. Moniruzzaman and Oluwaseun for the suggestions.
Priyanka Trivedi Hi! What did you use as your solvent? I am encountering the same problem. Please share what you did.
Hi Angelo,
We tried a variety of solvents ranging from chloroform, acetone, DMSO, methanol, hexane etc.Actually the solubility of wax in a particular solvent depends on the composition of wax.
For DPPH assay, we continued with methanol for our waxes with a bit of heating.
Please note the waxes are a bit difficult to work with and you may never find 100 % solubility in any solvent. You may have to check more than one solvent, different dilutions.
Priyanka Trivedi we are planning to remove it from our analysis as not to hamper the results! Thank you very much for your response
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