What concentrations of percoll is needed to form the two-phase percoll gradient, and which buffer is best used?

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Giuseppe Scapigliati

Try with Percoll 46% (density 1.07 g/cm3) in HBSS buffer

Adeola Folasade Odewusi-Ehigie

HBSS buffer? As in Hank's balanced salt solution? Doesn't dt contain calcium chloride? That's the same salt I should be careful of in my experiment. Can i use the PBS instead? It's what I am using to wash the cells anyway.