To produce a CRISPR Knock out in bacteria where my HDR (single-stranded and double-stranded) has the right homology arm of the left arm pair of 150, should HDR concentration be used for knocking?
15 December 2020 7,284 2 View
I am looking for a research paper about the mathematical or computational modelling of protein oxidation (caused by reactive oxygen species).. I would really appreciate that if someone helps me...
17 November 2020 2,340 3 View
I am looking for a research in which the finite element model was trained based on experimental results.
02 October 2020 9,277 19 View
18 September 2020 5,802 5 View
This design is to check for rupture in the steel element and the behavior of the bolt does not matter.
14 August 2020 6,580 2 View
I modeled a two-span concrete box girder bridge base on FHWA design specification, and now I recognize a problem in modeling because my first period is higher than I expected. With initial...
12 July 2020 415 3 View
I am trying to run my VUMAT code with a model in Abaqus/explicit. After that the run has been completed, I observed that the stresses have been evolved in only one element as shown in the attached...
27 May 2020 5,506 3 View
I have some raw DSC test data and I need to do baseline correction. I don't know how should I do it. I would appreciate it if someone can help me with this.
16 May 2020 8,273 7 View
Hello all, Actually, I am dealing with an issue that I can't get desirable results out of my curve-fitting problem. I used lsqcurvefit in the first place. Then, I added MultiStart to my code as...
30 April 2020 4,943 2 View
I'm researching the Self - Adaptive System of hospital systems.But I've had trouble figuring out how to do organic computing for self-adaptation in hospital systems.I am looking for an article or...
14 April 2020 1,743 3 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...
25 February 2021 9,054 3 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,109 5 View
The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...
15 February 2021 3,095 4 View
Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...
10 February 2021 6,090 3 View
I would like to evaluate the amount of reverse transcription (cDNA) on a 20um fixed mouse brain section. I am using random primers with RevertAid H Minus M-MuLV (reverse transcriptase). and would...
08 February 2021 1,957 4 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...
08 February 2021 2,032 2 View
1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.
04 February 2021 9,069 3 View
03 February 2021 221 2 View