I am trying to culture cells from a series of ovarian carcinomas, but it is the fibroblast population that is especially proliferative and time consuming to attend to. How do I get rid of them pre or post tumor cell seeding?
Hongjuan Cui
Suppose when you culture and split again and again, the ovarian cancer cell will take over and overgrowth rather than the fibroblast cell. In additional, you can dilute your cell and try to pick up sigel clone either in 96-well plate, so you will get ride of the fibroblast cell throughly. Good luck!
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