The 8ug/ml polybrene works well for the viral infection of multiple lung cancer cells in my hands. But recently I got a cell line isolated from mouse lung. 8ug/ml polybrene (with or without virus) can kill all the cells in 24 hours. Has anyone try to infect cells without polybrene? How low will the efficiency be?
Thomas Mühlenberg
Hi Yongming,
It really all depends on your cell lines. I sometimes forget to add polybrene to my lentiviral transductions but get very high infection rates. So if your cell line is very sensitive you may either use a drastically lower concentration or don't you any at all, it may just work very well.
Good luck!
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