Solution not converged, run continued at user's request in ansys apdl?

Why does my error file shows this warning message continuously but the solution is still going on? The error picture is attached

31 December 2017 4,188 1 View

What are the Constants of concrete of solid 65 element?

Hello, I want to use all the 9 constants of concrete for solid 65 . Where can get all the values for these parameters as the first four are always used so I have the idea about that but how the...

31 December 2017 5,593 3 View

How to rectify the error of too much element distortion in apdl?

I'm performing the analysis of shear wall with foundation 76*27*16 inches and the wall of dimension 48*144*4 inches in apdl. When the displacement controlled lateral cyclic loading is applied on...

31 December 2017 1,148 1 View

What could be the reason that the model takes more time to solve for nonlinear properties in apdl?

Hello, I am solving a RC wall model and I have observed that if I do the analysis for some time, then with only the linear properties, it is solved faster but as soon as I give a nonlinear...

31 December 2017 8,193 2 View

While modeling of RC shear wall that subjected to cyclic loading in ANSYS , analytical results differs from experimental results ?

I am working on a verification of RC shear wall subjected to cyclic loading using ANSYS APDL 14.50 but the results given by ansys are far different from that of the experimental results. - I used...

11 December 2017 3,551 2 View

How can I get reaction solution for by constrained nodes at every time step in ANSYS apdl?

I m performing analysis of shear wall which is constrained at the bottom and I have obtained the results. But in order to plot the reaction solution of the nodes at every time step, It has become...

11 December 2017 6,950 1 View

How do use bauschinger effect in ansys apdl?

I am applying a displacement controlled loading on a shear wall, I am using drucker prager parameters in the material properties, I want to capture the bauschinger effect. How can I do that in...

10 November 2017 9,458 2 View

Where will I find the values for the parameters of Drucker Prager model?

Can anyone suggest some papers where I would find values of cohesion, dialatancy angle of concrete to be used for drucker prager model in ansys?

08 September 2017 3,358 0 View

How to integrate the reaction forces on all nodes in ansys apdl?

For a cantilever beam with point force at end, I have analysed by apdl and got different reaction forces at different nodes (at the fixed point). how to get the single value of reaction force?

07 August 2017 9,095 6 View

What is convergence depended upon in ansys apdl?

05 June 2017 6,971 0 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View