How sensitive is qPCR in CFU/ml?

I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...

17 September 2019 7,790 4 View

How do I get transfection efficiency with a plasmid with an HA tag?

I want to use a plasmid that is HA tagged. My research is in vitro and I am bit confused on what to do. Thank you!

19 September 2018 1,130 3 View

How can you identify specific defined media that allows growth of newly isolated environmental microbes?

Thanks

13 April 2018 7,991 2 View

I have a large document, half citations from Mendeley and half from Endnote. How can I merge them?

The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...

10 February 2018 3,242 3 View

Is there a new standard/easy method of measuring protein protein interactions beyond yeast two-hybrid systems?

Without use of fancy equipment like ITC

09 June 2017 4,676 3 View

I want to determine whether SNPs have deleterious effects on proteins. How can I do this for multiple proteins simultaneously?

13 December 2016 8,234 3 View

Can someone help with the troubleshooting of an Elisa experiment?

I'm running a C-elisa for Brucella in swine samples and even though from the eye point of view the controls seem ok, the OD (at 450nm) of wells designated as conjugate (cc) are not getting...

05 November 2014 8,844 5 View

How long will Beta-Alanine (or any amino acid) last when stored in an aqueous solution?

I am using Beta-Alanine for a sensitive assay in which I supplement the medium of my organism with Beta-Alanine (in the micro/nano molar range). How long will a stock solution of Beta-Alanine,...

25 January 2014 7,467 1 View

Does anyone have experience with protein aggregating after purification and how can it be fixed?

I am trying to purify a new recombinant protein from e.coli. After I purify the protein and run on an SDS gel, I get the correct sized bands. When I run the sample on the FPLC, I get a vary small...

29 July 2013 7,919 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View