Sammy Pontrelli

14 Questions 6 Answers 0 Followers

Questions related from Sammy Pontrelli

Do cyanobacteria or algae need to be shaken during growth?

Growing cyanobacteria in flasks will definitely require some sort of aeration for growth, but in small culture volumes is this needed? If cyanobacteria are grown in small culture volumes (lets say...

21 January 2021 2,982 4 View

How sensitive is qPCR in CFU/ml?

I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...

18 September 2019 7,901 4 View

How can you identify specific defined media that allows growth of newly isolated environmental microbes?

Thanks

14 April 2018 8,066 2 View

I have a large document, half citations from Mendeley and half from Endnote. How can I merge them?

The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...

11 February 2018 3,344 3 View

What are useful online tools for cloning/genetic modification/molecular techniques?

I'm compiling a list

08 February 2018 5,928 3 View

Organic chemists of Research Gate: What do you look for in a molecule to determine if it's stable? (by itself or in aqueous solutions)

When trying to determine if a molecule is generally unstable or not, what do you look for? Also, is it possible to determine exactly how it will degrade? For example, if I have an aqueous solution...

15 July 2017 5,927 3 View

What effects can a synonymous mutation have?

If a metabolic gene has a synonymous mutation on it, what can that mean? Here are some hypotheses, please add to it if you think of any- 1. It affects RNA stability and degradation 2. It...

01 July 2017 8,389 4 View

What is biorxiv? And what do people use it for?

I don't really understand the concept of Biorxiv. What is it? Why do people use it? Are there any benefits or downsides of preprinting?

19 June 2017 6,574 25 View

Is there a new standard/easy method of measuring protein protein interactions beyond yeast two-hybrid systems?

Without use of fancy equipment like ITC

10 June 2017 4,753 3 View

I want to determine whether SNPs have deleterious effects on proteins. How can I do this for multiple proteins simultaneously?

There are online tools like SIFT, PANTHER, and PROVEAN that take snp inputs and then output whether that mutation will have an effect on the protein function. I have several genome sequences with...

14 December 2016 8,309 3 View

Lambda Red Recombinase Troubleshooting - pKD46. What am I doing wrong?

I have previously performed knockouts in E.coli using lambda red recombinase many many times. Now I can't do it anymore and I don't know what I'm doing wrong. Please help me! My standard protocol...

19 February 2016 8,737 6 View

How long will Beta-Alanine (or any amino acid) last when stored in an aqueous solution?

I am using Beta-Alanine for a sensitive assay in which I supplement the medium of my organism with Beta-Alanine (in the micro/nano molar range). How long will a stock solution of Beta-Alanine,...

26 January 2014 7,541 1 View

Does anyone have experience with protein aggregating after purification and how can it be fixed?

I am trying to purify a new recombinant protein from e.coli. After I purify the protein and run on an SDS gel, I get the correct sized bands. When I run the sample on the FPLC, I get a vary small...

30 July 2013 7,988 1 View

How will Coronavirus affect scientific goals and long term culture?

What do you think? Will people learn new technical skills? Will people learn how to collaborate better virtually? Will there be a surge of review papers? What are you doing?

01 January 1970 6,848 4 View