How do I get transfection efficiency with a plasmid with an HA tag?

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Dominique Liger

Hi there,

Transfection efficiency is the ratio between cells expressing the transfected protein and the total number of cells. So in your case it will be about detecting HA epitope (by IM I guess) and counting cells.

Anand Patwardhan

Hi,

Give more details, HA tags are for in vivo or cellular expression.

HA antibodies work pretty well.

Good luck...

Sammy Mustafa

Anand Patwardhan Dominique Liger Thank you for responding. I want to transfect the T98G cell line with a plasmid from a pseudogene. However, the plasmid description on Addgene mentioned that there is a HA (N terminal on backbone) tag. I was wondering if there is any way to get the transfection efficiency using this tagged protein, without the HA antibody would be preferable. Again, thank you so much.