How sensitive is qPCR in CFU/ml?

I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...

17 September 2019 7,790 4 View

How do I get transfection efficiency with a plasmid with an HA tag?

I want to use a plasmid that is HA tagged. My research is in vitro and I am bit confused on what to do. Thank you!

19 September 2018 1,130 3 View

How can you identify specific defined media that allows growth of newly isolated environmental microbes?

Thanks

13 April 2018 7,991 2 View

I have a large document, half citations from Mendeley and half from Endnote. How can I merge them?

The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...

10 February 2018 3,242 3 View

Is there a new standard/easy method of measuring protein protein interactions beyond yeast two-hybrid systems?

Without use of fancy equipment like ITC

09 June 2017 4,676 3 View

I want to determine whether SNPs have deleterious effects on proteins. How can I do this for multiple proteins simultaneously?

13 December 2016 8,234 3 View

How high is the limit of puromycin using in cell selection ?

I am looking for any papers that mentioned about using puromycin in cell selection after transfection. I already got the optimized of MTX and G418. Then I started cell selection with puromycin at...

20 October 2016 6,914 5 View

Can someone help with the troubleshooting of an Elisa experiment?

I'm running a C-elisa for Brucella in swine samples and even though from the eye point of view the controls seem ok, the OD (at 450nm) of wells designated as conjugate (cc) are not getting...

05 November 2014 8,844 5 View

How long will Beta-Alanine (or any amino acid) last when stored in an aqueous solution?

I am using Beta-Alanine for a sensitive assay in which I supplement the medium of my organism with Beta-Alanine (in the micro/nano molar range). How long will a stock solution of Beta-Alanine,...

25 January 2014 7,467 1 View

Does anyone work with an existential paradigm in end of life care or palliative care?

I'm looking for colleagues who combine both perspectives as I do in my work for networking and developing mutual research strategies.

07 August 2013 8,691 8 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View