How sensitive is qPCR in CFU/ml?

I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...

17 September 2019 7,790 4 View

How do I get transfection efficiency with a plasmid with an HA tag?

I want to use a plasmid that is HA tagged. My research is in vitro and I am bit confused on what to do. Thank you!

19 September 2018 1,130 3 View

I have a large document, half citations from Mendeley and half from Endnote. How can I merge them?

The large document has around 400 citations and it is in two sections. The first half is with Mendeley and the second half is with Endnote. Can I somehow take one of these halves and convert...

10 February 2018 3,242 3 View

Is there a new standard/easy method of measuring protein protein interactions beyond yeast two-hybrid systems?

Without use of fancy equipment like ITC

09 June 2017 4,676 3 View

I want to determine whether SNPs have deleterious effects on proteins. How can I do this for multiple proteins simultaneously?

13 December 2016 8,234 3 View

Can someone help with the troubleshooting of an Elisa experiment?

I'm running a C-elisa for Brucella in swine samples and even though from the eye point of view the controls seem ok, the OD (at 450nm) of wells designated as conjugate (cc) are not getting...

05 November 2014 8,844 5 View

How long will Beta-Alanine (or any amino acid) last when stored in an aqueous solution?

I am using Beta-Alanine for a sensitive assay in which I supplement the medium of my organism with Beta-Alanine (in the micro/nano molar range). How long will a stock solution of Beta-Alanine,...

25 January 2014 7,467 1 View

Does anyone have experience with protein aggregating after purification and how can it be fixed?

I am trying to purify a new recombinant protein from e.coli. After I purify the protein and run on an SDS gel, I get the correct sized bands. When I run the sample on the FPLC, I get a vary small...

29 July 2013 7,919 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How to consider effects of rapid warm adaptation in modeling of ecological impacts of warming?

I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...

15 February 2021 3,642 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View