In the picture derived from light microscopy, there are some red stains apart from the blue ones. I am wondering, what could it be?
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
Please help me in plotting the photon-magnon coupling plot as shown below
14 July 2024 1,632 1 View
Hi, My research will consist in examining the effect of compounds at concentrations of 10(-4)M, 10(-5)M, 10(-6)M, 10(-7)M, 10(-8)M on cells. I prepared 100 mM stocks of compounds in 100% DMSO....
21 April 2024 3,032 0 View
Good day Water, marine Biologist scientist, Environmental Engineers,and Industrial Ingineers, chemists and chemical Engineers experts I hope this message finds you well. I am currently serving as...
06 April 2024 3,681 4 View
how we can interpret it??
18 March 2024 4,915 2 View
amount of radiation in space
09 March 2024 3,855 0 View
I have done ph.x, q2r.x, matdyn.x and have got the output. But, while running plotband.x I am getting error like "error reading k point # 1"
03 March 2024 8,899 0 View
Atomic bomb surviours of (out of ~ 105500 or so reported) of Hiroshima and Nagashaki in Japan
02 March 2024 8,583 1 View
MALDI( Matrix Assisted Laser desorption Ionization) Matrix use in MALDI in positive mode and negative mode
18 February 2024 5,919 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...
10 August 2024 3,229 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View