What are the suitable blocking and fixation (inactive) buffer composition for bacterial flow cytometry analysis?

Vincent Chiang @Vincent_Chiang

10 October 2015 1 7K Report

I am going to use flow cytometry to analyze the bacterial surface protein.

Does anyone know about the best blocking and the fixation buffer ingredient for bacterial on this experiment?

Thank you.

Sun Yung

Never realised it was possible to analyse bacteria by flow but came across this which may help.

https://www.bdbiosciences.com/documents/Bacterial_Detection_Live_Dead.pdf

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Science method

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