How do I separate 2-5 KDa proteins on SDS-page?

Does anyone have experiences to separate 2 to 5 KDa protein in SDS-PAGE procedure? Thank you.

09 October 2015 3,992 6 View

What is the background fluorescent intensity of normal mouse serum?

I have worked on an experiment that I have to mix bacteria, mouse antiserum and DNA binding agent (like EtBr or AO). Then, kinetic detection of fluorescent intensity was measured at selective time...

09 October 2015 3,707 2 View

What are the suitable blocking and fixation (inactive) buffer composition for bacterial flow cytometry analysis?

09 October 2015 7,094 1 View

Could we use precipitate protein or the soluble protein to do a study?

When we refold the protein by reducing the denature buffer, we always suffer from protein precipitate. Is it ok for using the rest of the soluble form to do a bioactive experiment and animal study?

05 June 2013 3,796 3 View

MBGD analysis

If I have 10 strains genome of a bacteria from NCBI. Firstly, I want to analyze to find a list of genes that are homologous in these strains. (I have done this work on MBGD, but I don't know how...

31 December 2012 2,502 5 View

Does anyone have website suggestion for analyzing bacterial genome?

For example, signaling motif, T cell or B cell binding site, hydrophobic region, and so on. For reverse vaccinology.

10 November 2012 4,133 13 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View