Vincent Chiang

7 Questions 21 Answers 0 Followers

Questions related from Vincent Chiang

What are the suitable blocking and fixation (inactive) buffer composition for bacterial flow cytometry analysis?

I am going to use flow cytometry to analyze the bacterial surface protein. Does anyone know about the best blocking and the fixation buffer ingredient for bacterial on this experiment? Thank you.

10 October 2015 7,164 1 View

What is the background fluorescent intensity of normal mouse serum?

I have worked on an experiment that I have to mix bacteria, mouse antiserum and DNA binding agent (like EtBr or AO). Then, kinetic detection of fluorescent intensity was measured at selective time...

10 October 2015 3,771 2 View

How do I separate 2-5 KDa proteins on SDS-page?

Does anyone have experiences to separate 2 to 5 KDa protein in SDS-PAGE procedure? Thank you.

10 October 2015 4,060 6 View

Could we use precipitate protein or the soluble protein to do a study?

When we refold the protein by reducing the denature buffer, we always suffer from protein precipitate. Is it ok for using the rest of the soluble form to do a bioactive experiment and animal study?

06 June 2013 3,857 3 View

Does anyone have experience on Epitope prediction (both T & B cell)? What is your recommendation?

Epitope prediction have many tool online, but which one will be a generally suitable for polycolone antibody production (maybe for western blot)?

03 March 2013 239 5 View

MBGD analysis

If I have 10 strains genome of a bacteria from NCBI. Firstly, I want to analyze to find a list of genes that are homologous in these strains. (I have done this work on MBGD, but I don't know how...

01 January 2013 2,571 5 View

Does anyone have website suggestion for analyzing bacterial genome?

For example, signaling motif, T cell or B cell binding site, hydrophobic region, and so on. For reverse vaccinology.

11 November 2012 4,212 13 View