I have worked on an experiment that I have to mix bacteria, mouse antiserum and DNA binding agent (like EtBr or AO). Then, kinetic detection of fluorescent intensity was measured at selective time series. But I found that mix with bacteria and antiserum already have a high background fluorescent intensity (Ex 502 Em 525). Does anyone have any opinions on that issue? Thank you.
Hi Vincent,
I am not well versed with antiserum thing. But still i would like to give a suggestion regarding excitation wavelength. If you are exciting your mix, depending on the type of binding agent you may get low background antiserum fluorescent intensity. Because few nanometer changes in ex wavelength may change the fluorescent yield and spectra. Give a try. It may work.
Thanks Poonam,
Finally, I found the resolution that was to use another binding agent with different Ex/Em spectrum. Sometimes we have to take a rest and think the alternative way to do. Thank you.
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