Can someone share their protocol for culture of primary human adrenal cortical cells please.
Thank you in advance!
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Nothing
01 August 2024 755 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...
27 July 2024 201 0 View
Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...
24 July 2024 3,284 6 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
How to determine tannin content in fodder pellets?
22 July 2024 8,163 3 View
I want researchers who specialize in studying artificial intelligence in the field of science education and those related to it to give me information about the application of the...
18 July 2024 8,320 0 View
I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
07 July 2024 9,034 2 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,609 3 View
Receiving reviews of a paper (Tehovnik, Carvey et al. 2003) in which we electrically stimulated the visual cortex of primates to evoke saccadic eye movements under various behavioral conditions to...
19 June 2024 4,676 0 View
I have been trying to culture P. falciparum, but I see contamination after ~1 week of culturing. What could this organism possibly be (image attached)? The culture media consists of RPMI, HEPES,...
15 June 2024 5,515 5 View
I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...
12 June 2024 1,714 4 View
Hello everyone! I have been trying to develop a protocol for my thesis research which includes culturing and storing Bifidobacteria. I have come across many articles and studies related to the...
10 June 2024 4,152 1 View
I'm trying to culture the cell lines H69 and H69AR. Regarding the parental cell line, I'm finding my way in the handling, but with H69AR it's been troublesome. Currently, I'm culturing H69AR in...
05 June 2024 3,471 0 View
Hi Everyone! Currently, I am culturing the BM-MSCs, but it is taking very long, and the yield is very low. I am using DMEM+10% FBS+L-glutamine and I tried the Mesencult media as well. Please let...
02 June 2024 2,140 0 View
I am culturing HEK293 cells. When I plated these cells in a 96-well plate, the cells looked healthy and there were no precipitates. However, after I dosed them with the drug RITA, they developed...
30 May 2024 5,169 2 View
Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...
28 May 2024 1,398 0 View