- Hello Everyone,
- I have a bioactive small molecule that target MDA-MB-468 (TNBC cell line) cells and induces robust cytotoxicity, but not effective in neither TNBC nor other normal and cancer cells of different origin. I found a significant rescue of cell death with CAPE and Luteolin but not with other 4 NF-kB inhibitors I had used in combination with the small molecule. moreover, I found significant rescue in small molecule induced cytotoxicity in knockdown of P65 but not in other subunits of NF-kB. I look for nuclear localization of p65 through western blot and confocal and find no significant change in localization but confirmed disruption of IkBalpha-p65 complex when treated with the small molecule.
- So I'm excited about how I explain the small molecule induced cell death.
- Either NF-kB showing pro-apoptotic function after treatment, But how?
- Is there any cytosolic function of p65?
- What are the possible signaling pathways involved in cytotoxicity induced by p65?
- Any experiment suggestion that helps me to find out the mechanism involved.
Thanks.
Alexandr Chernov
Dear Krishan!
Please You look at following publications:
Article NF-κB p65 recruited SHP regulates PDCD5-mediated apoptosis i...
Nucleolar NF-κB/RelA mediates apoptosis by causing cytoplasmic relocalization of nucleophosmin
https://www.nature.com/articles/cdd201179
Article Apoptotic Pathway as the Therapeutic Target for Anticancer T...
Anticancer TCMs Through Inhibiting The NF-κB Pathway
https://www.nextprot.org/entry/NX_Q04206/
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Eddy Breemen
Hello Krishan,
Might be interesting to look at our assay that measures the kinase activity in your lysate, to help figure out what are the effects of your compound on signalling and uncover the mechanism. Read more at https://pamgene.com/science/
If you have any questions leave me a message at [email protected]
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