Can anyone suggest me something that could delay/inhibit C2C12 differentiation?

I am looking forward to a method that can delay/inhibit C2C12 differentiation.

07 August 2017 7,551 3 View

Which are some good publication on mechanics of C2C12 myogenesis?

I am currently working with C2C12 cells. I would like to address the membrane fluctuations of the cells through my studies. It would be a great help if anyone could suggest me some good reads on...

31 December 2016 7,856 0 View

What is the role of insulin differentiation medium of C2C12 myoblasts?

C2C12 cells on reaching desired confluency, are supplemented with differnetiation medium containing insulin. I wonder what is the role of insulin in myogenesis of C2C12 cells.

31 December 2016 2,254 2 View

Can we grow C2C12 cells on fibronecctin coated plastic dishes?

I am trying to differentiate the C2C12 myoblast cells to myotubes. I tried to grow the cells in glass bottom dishes. I found that the cells are getting elongated initiallly, but shrinking and...

10 November 2016 6,326 3 View

Why are the cells incubated at 4 degree Celsius while NBD staining?

I have been staining the cells(Hela and CHO) using NBD C6 ceramide. Why are the cells treated with NBD under 4 degree celsius ?

08 September 2016 338 1 View

What is the working mechanism of NBD C6 Ceramide?

I have been staining the Golgi Apparatus using NBD C6 Ceramide. I wonder how the dye specifically stains Golgi?

08 September 2016 6,101 1 View

How are the transfected nucleic acids transported to nucleus?

I am trying to do lipofection. I just want to know how the nucleic acids which are incorporated in to the cell by means of liposomes are transported in to the nucleus.

08 September 2016 3,116 1 View

Can anyone explain how to work with RNAhybrid in cygwin?

I am here to find the miRNA target sites. I could do that online via RNAhybrid web service. But I have no idea how to work with RNAhybrid in cygwin.

05 June 2014 5,185 1 View

What are actually novel miRNAs?

As a beginner in molecular genetics I have come across and got confused with the term novel-miRNA many times. But I can't figure out what it really is.

05 June 2014 7,279 1 View

Drawbacks of Lifeact in live cell imaging

LifeAct is a 17aa peptide sequence that could be conjugated with a fluorophore that could bind actin. Though Lifeact is being widely used for live cell imaging, it is reported that there are...

01 January 1970 8,802 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View