I am tying to transfect CHO cells using MTag Golgi GFP using lipofection. I wonder how do OptiMEM and P3000 reagent work in lipofection?
I am looking forward to a method that can delay/inhibit C2C12 differentiation.
07 August 2017 7,662 3 View
I am currently working with C2C12 cells. I would like to address the membrane fluctuations of the cells through my studies. It would be a great help if anyone could suggest me some good reads on...
31 December 2016 7,931 0 View
C2C12 cells on reaching desired confluency, are supplemented with differnetiation medium containing insulin. I wonder what is the role of insulin in myogenesis of C2C12 cells.
31 December 2016 2,335 2 View
I am trying to differentiate the C2C12 myoblast cells to myotubes. I tried to grow the cells in glass bottom dishes. I found that the cells are getting elongated initiallly, but shrinking and...
10 November 2016 6,431 3 View
I have been staining the cells(Hela and CHO) using NBD C6 ceramide. Why are the cells treated with NBD under 4 degree celsius ?
08 September 2016 458 1 View
I have been staining the Golgi Apparatus using NBD C6 Ceramide. I wonder how the dye specifically stains Golgi?
08 September 2016 6,227 1 View
I am trying to do lipofection. I just want to know how the nucleic acids which are incorporated in to the cell by means of liposomes are transported in to the nucleus.
08 September 2016 3,197 1 View
I am here to find the miRNA target sites. I could do that online via RNAhybrid web service. But I have no idea how to work with RNAhybrid in cygwin.
05 June 2014 5,307 1 View
As a beginner in molecular genetics I have come across and got confused with the term novel-miRNA many times. But I can't figure out what it really is.
05 June 2014 7,358 1 View
LifeAct is a 17aa peptide sequence that could be conjugated with a fluorophore that could bind actin. Though Lifeact is being widely used for live cell imaging, it is reported that there are...
01 January 1970 8,873 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View