Can anyone suggest me something that could delay/inhibit C2C12 differentiation?

I am looking forward to a method that can delay/inhibit C2C12 differentiation.

07 August 2017 7,551 3 View

Which are some good publication on mechanics of C2C12 myogenesis?

I am currently working with C2C12 cells. I would like to address the membrane fluctuations of the cells through my studies. It would be a great help if anyone could suggest me some good reads on...

31 December 2016 7,856 0 View

What is the role of insulin differentiation medium of C2C12 myoblasts?

C2C12 cells on reaching desired confluency, are supplemented with differnetiation medium containing insulin. I wonder what is the role of insulin in myogenesis of C2C12 cells.

31 December 2016 2,254 2 View

Can we grow C2C12 cells on fibronecctin coated plastic dishes?

I am trying to differentiate the C2C12 myoblast cells to myotubes. I tried to grow the cells in glass bottom dishes. I found that the cells are getting elongated initiallly, but shrinking and...

10 November 2016 6,326 3 View

What are the roles of Opti MEM and P3000 reagent in lipofectamine trasfection?

08 September 2016 5,694 0 View

Why are the cells incubated at 4 degree Celsius while NBD staining?

I have been staining the cells(Hela and CHO) using NBD C6 ceramide. Why are the cells treated with NBD under 4 degree celsius ?

08 September 2016 338 1 View

What is the working mechanism of NBD C6 Ceramide?

I have been staining the Golgi Apparatus using NBD C6 Ceramide. I wonder how the dye specifically stains Golgi?

08 September 2016 6,101 1 View

How are the transfected nucleic acids transported to nucleus?

I am trying to do lipofection. I just want to know how the nucleic acids which are incorporated in to the cell by means of liposomes are transported in to the nucleus.

08 September 2016 3,116 1 View

What are actually novel miRNAs?

As a beginner in molecular genetics I have come across and got confused with the term novel-miRNA many times. But I can't figure out what it really is.

05 June 2014 7,279 1 View

Drawbacks of Lifeact in live cell imaging

LifeAct is a 17aa peptide sequence that could be conjugated with a fluorophore that could bind actin. Though Lifeact is being widely used for live cell imaging, it is reported that there are...

01 January 1970 8,802 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Please suggest me the simplest way to understand the in-silico analysis of any gene?

I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?

22 February 2021 8,879 2 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Is there a Constitutive model for concrete with Sea-Sand?

Hi I am running a simulation in Abaqus for a concrete beam. Its fine aggregate replaced with sea-sand. I was wondering if I could find studies that measured the ultimate strain of concrete made...

22 February 2021 6,409 2 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View