Athul M Sivan

11 Questions 2 Answers 0 Followers

Questions related from Athul M Sivan

Can anyone suggest me something that could delay/inhibit C2C12 differentiation?

I am looking forward to a method that can delay/inhibit C2C12 differentiation.

08 August 2017 7,613 3 View

What is the role of insulin differentiation medium of C2C12 myoblasts?

C2C12 cells on reaching desired confluency, are supplemented with differnetiation medium containing insulin. I wonder what is the role of insulin in myogenesis of C2C12 cells.

01 January 2017 2,300 2 View

Which are some good publication on mechanics of C2C12 myogenesis?

I am currently working with C2C12 cells. I would like to address the membrane fluctuations of the cells through my studies. It would be a great help if anyone could suggest me some good reads on...

01 January 2017 7,900 0 View

Can we grow C2C12 cells on fibronecctin coated plastic dishes?

I am trying to differentiate the C2C12 myoblast cells to myotubes. I tried to grow the cells in glass bottom dishes. I found that the cells are getting elongated initiallly, but shrinking and...

11 November 2016 6,386 3 View

How are the transfected nucleic acids transported to nucleus?

I am trying to do lipofection. I just want to know how the nucleic acids which are incorporated in to the cell by means of liposomes are transported in to the nucleus.

09 September 2016 3,156 1 View

Why are the cells incubated at 4 degree Celsius while NBD staining?

I have been staining the cells(Hela and CHO) using NBD C6 ceramide. Why are the cells treated with NBD under 4 degree celsius ?

09 September 2016 407 1 View

What is the working mechanism of NBD C6 Ceramide?

I have been staining the Golgi Apparatus using NBD C6 Ceramide. I wonder how the dye specifically stains Golgi?

09 September 2016 6,176 1 View

What are the roles of Opti MEM and P3000 reagent in lipofectamine trasfection?

I am tying to transfect CHO cells using MTag Golgi GFP using lipofection. I wonder how do OptiMEM and P3000 reagent work in lipofection?

09 September 2016 5,752 0 View

What are actually novel miRNAs?

As a beginner in molecular genetics I have come across and got confused with the term novel-miRNA many times. But I can't figure out what it really is.

06 June 2014 7,327 1 View

Can anyone explain how to work with RNAhybrid in cygwin?

I am here to find the miRNA target sites. I could do that online via RNAhybrid web service. But I have no idea how to work with RNAhybrid in cygwin.

06 June 2014 5,266 1 View

Drawbacks of Lifeact in live cell imaging

LifeAct is a 17aa peptide sequence that could be conjugated with a fluorophore that could bind actin. Though Lifeact is being widely used for live cell imaging, it is reported that there are...

01 January 1970 8,840 2 View