I am trying to differentiate the C2C12 myoblast cells to myotubes. I tried to grow the cells in glass bottom dishes. I found that the cells are getting elongated initiallly, but shrinking and becoming round in shape later. I used the differentiation medium supplemented with 2% Horse Serum and 1% of 1um insulin.
Hi Athul,
the best is to use chamber slides, because whenyou remove glass coverslip from the dishes this process breaks myotube.
C2C12 can differentiate avoiding serume depletion upon confluence reaching, and we performed several time differentiation in chamber slides without coating agents.
Good luck
Hi Cesare,
What kind (maybe “brand”) of chamber slides you used?
Thank you!
Hi Osvaldo,
we use always BD-Falcon plasaticware and as well for culture slide and chamber clide.
Good luck
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