I am currently working with C2C12 cells. I would like to address the membrane fluctuations of the cells through my studies. It would be a great help if anyone could suggest me some good reads on mechanical studies of C2C12 cells
I am looking forward to a method that can delay/inhibit C2C12 differentiation.
07 August 2017 7,662 3 View
C2C12 cells on reaching desired confluency, are supplemented with differnetiation medium containing insulin. I wonder what is the role of insulin in myogenesis of C2C12 cells.
31 December 2016 2,335 2 View
I am trying to differentiate the C2C12 myoblast cells to myotubes. I tried to grow the cells in glass bottom dishes. I found that the cells are getting elongated initiallly, but shrinking and...
10 November 2016 6,431 3 View
I have been staining the cells(Hela and CHO) using NBD C6 ceramide. Why are the cells treated with NBD under 4 degree celsius ?
08 September 2016 458 1 View
I am tying to transfect CHO cells using MTag Golgi GFP using lipofection. I wonder how do OptiMEM and P3000 reagent work in lipofection?
08 September 2016 5,785 0 View
I have been staining the Golgi Apparatus using NBD C6 Ceramide. I wonder how the dye specifically stains Golgi?
08 September 2016 6,227 1 View
I am trying to do lipofection. I just want to know how the nucleic acids which are incorporated in to the cell by means of liposomes are transported in to the nucleus.
08 September 2016 3,197 1 View
I am here to find the miRNA target sites. I could do that online via RNAhybrid web service. But I have no idea how to work with RNAhybrid in cygwin.
05 June 2014 5,307 1 View
As a beginner in molecular genetics I have come across and got confused with the term novel-miRNA many times. But I can't figure out what it really is.
05 June 2014 7,358 1 View
LifeAct is a 17aa peptide sequence that could be conjugated with a fluorophore that could bind actin. Though Lifeact is being widely used for live cell imaging, it is reported that there are...
01 January 1970 8,873 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...
31 July 2024 9,859 0 View