Effect of Lycopene on prostate Cancer cell line, LNCaP (Cell Viability, restoration of lost Glutathione-S-Tranferases enzyme in cells, DNA methylation etc).
According to the NCI data, lycopene (CAS 502-65-8, NSC 407322) at concentrations up to 100 uM did not significantly inhibit growth of 60 different cancer cell lines treated for 48 hours.
http://tinyurl.com/nbb6qub
Among these cell lines two were prostate cancer cell lines DU-145 and PC-3. While LNCaP cells are androgen-dependent and the two prostate cancer cell lines included in the panel were androgen-independent, I would say that these results warrant some skepticism with respect to the effect of lycopene on cancer cell lines in general. Appreciable effects on cell proliferation/cell death in vitro may require much higher concentrations, which may be above the level of biological relevance.
Thank you so much Roman for your kind concern answering it. I find it quite interesting will try working on it , may be increasing the cell treatment time from 48 hr to 72 or 144 hrs might give significant results while maintaining the concentration at biological level(100 uM).especially on cell proliferation and induction of Glutathione S tranferses enzyme which is usually lost in the cell lines during onset of PC.
You are welcome Vaibhav! Some evidence suggests the role of GST polymorphisms and/or repression of its expression in prostate cancers and lycopene reportedly increases GSH level and activity of GSH-dependent enzymes, including GSTs. I would expect that changes in GSH and associated enzymes can play role in prostate carcinogenesis rather than affect the proliferation and/or viability of prostate cancer cells. So activation of GSTs may inhibit certain steps of malignant transformation in prostate epithelial cells, but may not affect viability of cells that are already malignant. In fact, activation of GSH and GSTs in cancer cells may, under some circumstances, contribute to their resistance against some anticancer drugs and radiotherapy. Consequently, it would be better to test lycopene in some model of prostate carcinogenesis (in vitro or in vivo) rather than on prostate cancer cells in vitro or established tumors in vivo.
Thank you so much Roman once again for such a detailed n well explained answer I got your point .will further try to implement it .Really appreciate your time and kind consideration.
Many Thanks.
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