I am incrementally adopting cell lines of rice to osmotic stress using PEG media. Some of the cells in the suspension started dying when I transferred cells from 5% PEG media to 10% PEG. Any possible ideas why?
The only think is coming into my mind is cytotoxicity. Maybe that concentration is too strong for your cells. As suggestion check in the literature.
Claudia
We have performed tolerance studies with human cells and observed that for PEG concentrations of 10 and 20% the viability and fold increase were very low. Our cells were however able to survive in 2, 4 and 6% PEG. We also observed that the molecular weight of the PEG was also important. I believe that the reason resides on a change of the osmotic pressure and water concentration (PEG is preferentially hydrated).
Rice cells have capacity to adopt up to 20% PEG. Cells lines stable at 5% PEG are transferred the to 10% PEG so there must be other reasons. PEG is not taken up by the cells due to its high mol weight, it just give osmotic stress to the cells
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