Farhan Ullah

6 Questions 7 Answers 0 Followers

Questions related from Farhan Ullah

Can saccharomyces cerevisiae CRISPR/Cas9 vector such as pML104, can be used for gene editing in Hansenula polymorpha?

I am curious if anybody used CRISPR/Cas9 vector pML104 for gene editing in Hansenula polymorpha.

16 August 2022 6,281 0 View

Is there any difference in transformation methodology for Pichia pastoris and Hansenula polymorpha?

It's my first time working with yeast. I need to find expression plasmids and a transformation protocol for H. polymorpha. I found a protocol for transforming P. pastoris, but I'm not sure if it...

10 July 2022 9,605 0 View

Can antibodies that come in serum format be used directly in ChIP, or should they be purified before use?

Hello everyone, I am performing ChIP in plant tissues. I bought a ChIP grade antibody from a company but it came in serum format (unpurified). My question is, do I need to purify it first or do I...

11 August 2020 4,650 2 View

Can anyone suggest a protocol for plasma membrane lipid extraction from rice cell lines?

I want to analyze the saturated and unsaturated fatty acids in different cell lines of rice.

30 August 2012 3,253 2 View

What are the possible reasons for cells dying in suspension culture containing 10% Polyethylene Glycol (PEG)?

I am incrementally adopting cell lines of rice to osmotic stress using PEG media. Some of the cells in the suspension started dying when I transferred cells from 5% PEG media to 10% PEG. Any...

12 July 2012 4,290 4 View

Best bioinformatics software for designing specific primers and probes.

What is the best bioinformatics software for designing specific primers and probes? I am currently working on the design of 16S rRNA probes for bacterial identification. While Primer Premier is my...

01 January 1970 9,989 4 View