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I am curious if anybody used CRISPR/Cas9 vector pML104 for gene editing in Hansenula polymorpha.
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It's my first time working with yeast. I need to find expression plasmids and a transformation protocol for H. polymorpha. I found a protocol for transforming P. pastoris, but I'm not sure if it...
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Hello everyone, I am performing ChIP in plant tissues. I bought a ChIP grade antibody from a company but it came in serum format (unpurified). My question is, do I need to purify it first or do I...
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