Does anyone have or know about a protocol for isolating microvessels from mouse brain to study tight junction proteins?

There are different protocols for microvessel isolation from mice or rat. Which protocol should I follow to isolate microvessels from mouse brain so that I can evaluate different tight junction...

05 January 2021 696 2 View

How can I use an hybridization of ontology and machine learning for text summarization ?

my work consists of creating a system for text summarization in a specific domain(crisis management) using a hybridization of ontology and machine learning algorithme ,my question is how to use...

23 February 2020 8,327 2 View

Gauge Pressure and Operating pressure on combustion system?

Hi all, Please I have a question about the pressure: I am working on combustion, and I want to study the effect of pressure in my geometry, I couldn't understand where sould I put the pressure for...

14 February 2020 7,977 3 View

What will happen if I add the NO models (NO thermal and NO prompt) for the flamelet model using GRI mech? How Fluent works in this case?

Hi everyone, The GRI mechanism contain all the reactions, and species of NOx, and in the Fluent we can also find the NO models ( prompt, thermal and N2O..), when I added the models I found a big...

22 December 2019 3,785 5 View

Is there a difference in binding site when comparing anti-CD279 (PD-1) clones EH12.2H7 and EH12.1?

Hi all, I would like to know if these clones of anti-PD-1 antibodies (EH12.2H7 and EH12.1) share the exact binding site. Thank you in advance for your help, Sabrina

19 March 2019 4,908 3 View

Is the title and goal of my project suitable?

I am starting works on a project with current working title: Gender Roles in African Philosophy: Appraising the place of women in African traditional society. A Case Study on Setswana and...

20 January 2019 2,161 4 View

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions?

How to fix the Error: The mesh file exporter could not resolve cyclic dependencies in overlapping contact regions. Please try to Repair Overlapping Contact Regions.

01 December 2018 7,320 3 View

What to use for correlation between ordinal scales?

Hello, I have a variable "firm's results" which is an ordinal scale and 5 variables for direct marketing, each of them in ordinal scale too. I know that I can use Pearson if I want to know the...

05 November 2018 1,535 3 View

I have tilt in my geometry, so Is there any way for doing Line moving with the flow ?

Dear professors and research scholars, I have a query for the code Fluent My geometry is not symmetric, I have tilt in my geometry so Is there any way for doing Line moving with the flow !!!...

19 February 2018 8,538 4 View

I have a problem with my results from FLUENT when I imported in ORIGIN, I have other lines! Does that mean the problem with the mesh or secondary air?

Dear professors and research scholars, Attached is a figure showing the distribution of the temperature along the central axis of combustor Fuel, I have problems with my results, When I put it in...

09 February 2018 738 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View