Sabrina Helmold Hait

6 Questions 5 Answers 0 Followers

Questions related from Sabrina Helmold Hait

Do I lose IL-12 Receptor expression on cultured cells?

Dear all, I am trying to set up a culture assay for pSTAT4 expression induced by IL-12 and for that I will need to rest my cells to improve the expression of some other markers. My question is,...

29 February 2024 8,944 0 View

What are the phenotypic markers expected on MOLT-4 CCR5 cell?

Dear RG community, I have been trying to standardize a viral outgrowth assay using MOLT-4 CCR5 cells. In that sense, as we want to compare the assay with PBMC standard protocol, we are interested...

28 October 2020 2,603 2 View

Why OX40 is presenting poor up-regulation in Env-specific T cells in PBMCs of rhesus macaques?

I have been using the CD25/OX40 AIM assay for identification of SIV-specific CD4 T cells in rhesus macaques in T cells stimulated with 1mg/ml of Env pooled peptide. I used this concentration to...

16 October 2019 8,096 0 View

Is there a difference in binding site when comparing anti-CD279 (PD-1) clones EH12.2H7 and EH12.1?

Hi all, I would like to know if these clones of anti-PD-1 antibodies (EH12.2H7 and EH12.1) share the exact binding site. Thank you in advance for your help, Sabrina

20 March 2019 5,052 3 View

Is it common to see a lot of insoluble particles when adding water to Horseradich peroxidase-conjugated IgG?

I ordered a horseradish peroxidase-conjugated IgG antibody from Nordic Immunology (polyclonal goat atiserum to rhesus monkey- code GAMon/SC/PO) and it did not seem to dissolve in distilled water....

27 February 2019 9,174 2 View

Is it possible to perform two rounds of macs beads isolation?

I would like to isolate a subpopulation of T cells using magnetic beads. Is it possible to perform two positive selections in a row using macs beads and still get functional cells for culture?

19 November 2017 6,167 3 View