Dear all,
I am trying to set up a culture assay for pSTAT4 expression induced by IL-12 and for that I will need to rest my cells to improve the expression of some other markers. My question is, by resting the cells overnight, will I lose IL-12 beta 2 receptor on T cells and/or NKs due to internalization? I am planing to use complete RPMI without any cytokine for the assay.
Many thanks for any help!
Sabrina