What parameters I should use to test the activity and accuracy of my compound against breast cancer in albino rats and cell lines? I just want to figure out everything that can be done to screen my compound against breast cancer. So could any one suggest about this?
One of the easiest experiments to test the citotoxicity of compounds is the sulphorodamine B assay, this is a NIH accepted assay and is a cheaper experiment to anwer your question. You can seed 8.000 cells/well in a 96-well plate, then breake and fix your cells with TCA50%, stain your plate-atached proteins with SRB and then solubilize them to mesure the protein content (proportional to number cells) by spectrophotometry. Ther exist a wide number of papers and protocols to performe the experiment.
Other strategies consist in the evaluation of the inhibitory effect on cell proliferation. For example using the BrdU incorporation assay. You can look for a kits.
Other easy assay is the wound-healing assay. You can measure how your cells can grow up in a line that you have previously generated using a tip. There are also automated methods for this porpose.
I hope to help you in your question.
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