could any one suggest me a good article which carries the treatment period and after how many hours should i kill the rats by cervical dislocation
protective effect of medicinal plant in formalin induced paw edema later followed by pure phytochemicals and want to analyse tissue also
markers i want to test is cox-2 and il-6
What are you using to cause the oedema? And what are you looking at in the tissue?
The treatment period and sacrifice time in acute inflammation varies based on model of inflammation. Which model of inflammation will you be using? Please explain what you mean by "treatment period". The duration of treatment will vary significantly based on type of treatment. Also, what, if anything, will you be measuring? And will you be looking at tissues after euthanasia? If so, what markers will you be looking at? More information is needed in order to answer your question.
If you are using the formalin model, which phase are you more concerned with? Phase I or II?
Formalin is nasty stuff and probably causes necrosis. Could you use carrageenan instead? The carrageenan paw oedema swelling response can be measured after 3 and 5h, it reaches a maximum at about 8h, but then has completely resolved by 24h. This model is used to test drug molecules in pharmaceutical companies. I suggest dosing 1-2 hours prior to the injection of 100ul lambda carrageenan in to the plantar surface of the rat paw (depends on the compound PK). Swelling can be assessed at 3 h and then at 5h you can also harvest tissue from the paw to look at PGE2 production or similar (fluid can be collected by squeezing the tissue). Use a NSAID or COX-2 inhibitor as a positive control. Check the literature for suitable drugs and doses.
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