I tried to transfect NIH3T3 with pIRESneo-GFP-Protein using XtreamGene HP and X9. However, it didn't work. Has anybody experience transfecting 3T3?

I tried using 2ug of plasmid and different ratios of XtreamGene (HP or X9) 3:1, 6:1. The efficiency of transfection is too low. In addition, selected clones died 24 or 48 h post-selection.

02 February 2013 4,966 13 View