Please, kindly help me with an optimized method of isolation of lactoferrin using CM-Sephadex-C50. Please, including the method of sephadex preparation from it solid form .
Instructions for use of CM Sephadex C50
https://cdn.cytivalifesciences.com/api/public/content/digi-13938-pdf
I have a response variable called skin yellowness, which I will measure via a scored color chart, whereby 1 is pale yellow and 15 is orange. I'm not sure if this counts as an ordinal variable,...
11 August 2024 4,793 1 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds
04 August 2024 3,019 3 View
Hi, I want to study the temperature and pH stability of lactoferrin, so I performed Circular Dichroism (CD) of lactoferrin at different pH (pH 1.2 and pH 2.0) and temperatures but observed no...
26 February 2024 2,947 1 View
I want to quantify lactoferrin that is released from liposomes.
03 December 2023 8,794 0 View
Lactoferrin is a natural antimicrobial in raw milk. I'm going to use this substance as food preservation.
23 September 2022 4,149 1 View
Is There any method to determine Lactoferrin in cheese using RP-HPLC, C18 column & UV Detector ? kindly share the same
05 June 2019 2,502 1 View
I'm working on Whey proteins isolation and mass spectrometry analysis. I have been able to purify most of them, but lactoferrin and immunoglobulins are hard to isolate. I'm using high q and high s...
02 March 2019 5,855 4 View
Good day! I have been reading on studies regarding the antibacterial properties of breast milk and its bacterial composition. It then crossed my mind, how exactly do these bacteria in breast milk...
01 February 2018 6,412 0 View
I will do heat treatment on lactoferrin in milk and would like to see the change in iron binding capacity. I would love to get a way of doing this using a spectrophotometer.
07 June 2017 3,609 1 View
Celia Conesaa, Lourdes Sáncheza, , , Carmen otab, María-Dolores Péreza, Miguel Calvoa, Sebastien Farnaudc, Robert W. Evansd: Isolation of lactoferrin from milk of different species: Calorimetric...
04 May 2017 3,928 1 View
Hello I have several questions: can anyone introduce me about these question? 1- Does interaction between of chimer lactoferricin -lactoferampin and cell membrane has critical role in...
20 January 2017 5,754 1 View
31 December 2016 7,430 1 View