I'm working on Whey proteins isolation and mass spectrometry analysis. I have been able to purify most of them, but lactoferrin and immunoglobulins are hard to isolate. I'm using high q and high s 1 ml macro prep columns. Any help would really be appreciated.
Hello
I have not purified it personally but can suggest going for heparin sepharose resin as affinity for lactoferrin. Otherwise, you may use the residual feed (after isolating other proteins) and then try tweaking the elution. Going for weaker IEX resins such as DEAE along with pH and conductivity adjustment of feed may also be looked into. Using salt precipitation may also help.
Please look at the following below links which may help you in your analysis:
https://www.researchgate.net/publication/328495598_Isolation_and_Purification_of_Whey_Protein_Lactoferrin_and_Evaluation_of_its_Antibacterial_Activity
https://www.arca.fiocruz.br/bitstream/icict/13586/2/Direct%20capture%20of%20lactoferrin%20from%20cheese.pdf
http://che.zju.edu.cn/biomass/Upfile/201308/22/1052.pdf
Thanks
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