How can I measure the iron-binding capacity of lactoferrin (whey milk protein) after doing heat treatment?

08 June 2017 1 4K Report

I will do heat treatment on lactoferrin in milk and would like to see the change in iron binding capacity. I would love to get a way of doing this using a spectrophotometer.

Paul Milham

Does it bind Fe (II) or Fe (III) ? Are you suggesting that there is new, unsatisfied Fe binding capacity? If so, there may be a competitive assay where xs of a coloured Fe complex is added and the loss of Fe to the milk protein registers as a decrease in absorbance.

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