What are the most reliable reference genes "endogenous controls" to select in order to normalize real time PCR gene expression analysis?

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Mira Ali Bosso

thanks Mahdy alot

all the best

Sindhu Jacob

Hi Mira,

18S is a good one

Greta Sokoloff

I've used B-Actin from RNA extracted from brain tissue. I am sure the source of the RNA will impact the one you want to use. I found this post helpful http://openwetware.org/wiki/Choosing_reference_genes_for_qPCR_normalisation

Like Mohamed mentioned you should try a couple, I also design 2 primer pairs for each gene and pick the more efficient pair before I run the experiment.