How to call both UMAT and UEL together in Abaqus?

I have a model with bulk material (matrix) and inclusions and cohesive interface elements between both. For the bulk material, I have implemented a UMAT and for the cohesive interface elements, I...

20 February 2021 1,286 2 View

Iam looking for articles on mycobacterium tuberculosis?

Hellow my fellows Iam working on Real time PCR technique for detection the gene who is responsiable on Tuberculosis as well as the AFB stain so i hope that any one have worked on the same project...

02 December 2020 2,123 3 View

I am looking for the dataset to test the algorithms for Radar Emiiter Classification?

I am working on Radar Emitter Classifiers and I want test my codes. unfortunately I'm unable to find a dataset to test my algorithms. I'll be glad if someone can help me with this...

04 November 2020 3,988 3 View

Precipitation of sugar contents from Fruit extract?

I have to perform In-vitro and In-vivo anti-diabetic activities on 99%, 70% and 50% ethanolic fruit extracts but I need to precipitate sugar contents first. Can anyone help me with the protocols...

18 October 2020 6,697 5 View

Anyone interest to apply, please go on?

04 October 2020 1,239 2 View

Can anyone please answer the issue mentioned below regarding PCR?

Currently, i am using PCR Machine of BioRAD C1000 Touch, I am having issue regarding PCR tubes, there is melting of tubes, i have tried to correct this error, I am using lid temperature as 105...

08 June 2020 2,044 19 View

A protocol to couple primary and secondary antibodies prior to flow cytometry staining?

Hi, I am interested in staining cells in human whole blood by Flow Cytometry. As I have to use a CD-19 FC-IgG1 primary and then come up with anti-human IgG1secondary to detect the marker of...

08 June 2020 7,160 3 View

Good Morning, Could we detect apoptosis or necrosis of cells after their implantation in vivo and on fixed sections of tissue?

Any Immunohistochemistry markers?? Thanks in advance.

30 May 2020 6,206 10 View

Which column indicates degraded DNA?

I'm in an introductory cell and molecular biology lab. This is a DNA gel electrophoresis. I've read that degraded DNA is indicated by smears (column 3) and also by a tight high molecular weight...

25 May 2020 6,937 24 View

What is the effect of curing temperature on mass loss of concrete at elevated temperatures?

can anyone has gone through some research paper or personal experience regarding study of elevated temperature performance of concrete, prepared under different curing regimes... I was looking for...

12 February 2020 8,316 5 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View