A protocol to couple primary and secondary antibodies prior to flow cytometry staining?

Hi, I am interested in staining cells in human whole blood by Flow Cytometry. As I have to use a CD-19 FC-IgG1 primary and then come up with anti-human IgG1secondary to detect the marker of...

08 June 2020 7,160 3 View

Good Morning, Could we detect apoptosis or necrosis of cells after their implantation in vivo and on fixed sections of tissue?

Any Immunohistochemistry markers?? Thanks in advance.

30 May 2020 6,206 10 View

Which column indicates degraded DNA?

I'm in an introductory cell and molecular biology lab. This is a DNA gel electrophoresis. I've read that degraded DNA is indicated by smears (column 3) and also by a tight high molecular weight...

25 May 2020 6,937 24 View

Is there an implemantation in java or matlab of hidding assolcation rule?

I need an implementation for my proposed algorithm.

25 May 2019 9,049 3 View

Hello Any idea on stainings that are not absorbed by the hydrogel ?

Any idea on stainings that are not absorbed by the hydrogel (Have tried toluidine, alcian blue, safranin-O, but they all get absorbed, despite the gel having cells or not). Perhaps non-aqueous...

04 February 2019 6,281 2 View

How could I analyze GFAP immunostained tissue?

I need analyze GFAP immunofluorescence in mouse brain tissue, how can I perform the analysis using ImageJ? I'm looking for neuroinflammation marks.

10 July 2017 8,213 3 View

Can I determine the thickness of thin film using ftir?

currently I have experiment about deposition carbon on the glass substrate. I have problem to solve from my experiment. can you help me? how must I do to determine the thickness of my thin film...

24 August 2016 8,352 3 View

Large-scale reaction with TnT T7 quick coupled transcription translation system (Promega)?

I wonder if the TnT T7 quick coupled transcription translation system (Promega) works in a large-scale reaction. They suggest a final volume of 50 ul and I would need a final volume reaction of...

17 August 2015 8,270 1 View

Does anyone know if there are any research groups looking for online researchers for International projects on Linguistics?

I would be glad to collaborate in International/European projects but they are hard to find and mainly if you can collaborate only online. My research expertise and skills are basically:...

27 May 2015 9,870 16 View

What are the most reliable reference genes "endogenous controls" to select in order to normalize real time PCR gene expression analysis?

The RNA samples are extracted from whole blood

20 October 2013 2,630 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View