Is a shortening of the telomeres one of the limitations?
There are lots of such mechanisms. You should check the rate of gases, the serum concentration in culture medium and other factors that can affect the senescence
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04 May 2015 5,566 0 View
We are using arteries from humans for myographical studies, but we have problems during contraction when we use phenylphrine (PE)? But, the arteries contract normally when we use KCl.
14 October 2014 8,913 3 View
I have a problem in the nuclear staining of the cells.
04 May 2014 5,099 1 View
01 May 2014 7,419 1 View
25 April 2014 1,365 1 View
Can it happen with continuous passage of pure cell culture ? Can the cell change its features?
03 April 2014 2,381 6 View
I have to make differential diagnosis between manage, ringworm, and parakeratosis in camels and I need some references.
03 April 2014 6,005 8 View
The source of action potential in the heart is the pacemaker cells of SA node. But, I am asking about the pacemaker cells in the brain (cerebrum), as in, what is the first cell that switches-on...
26 February 2014 9,277 22 View
Any one have interest to publish in a new journal in veterinary medicine and research it is a free online journal http://mirrorofresearchinveterinarysciencesandanimals.com/issue3.html.
08 September 2013 2,267 12 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View