I have a problem in the nuclear staining of the cells.
What is your pretreatment procedure?
Gamma Ray Spectrometery technique is a common technique that is used to determine activity concentration of isotopes. Could you please list other techniques which are used for measuring activity...
20 October 2018 7,823 18 View
I am using linear form of Redlich- Peterson Isotherm. There is a constant term A that need to be find in the equation. What are the possible ways of finding the constant term?
21 December 2015 2,356 8 View
?
04 May 2015 5,664 0 View
We are using arteries from humans for myographical studies, but we have problems during contraction when we use phenylphrine (PE)? But, the arteries contract normally when we use KCl.
14 October 2014 9,012 3 View
I am asking for estimating the location parameter of logistic distribution by Bayesian methods. I am in need of a proper prior distribution for µ , and how to know the prior is Normal ? or...
03 September 2014 4,854 3 View
I need some explanations about patch and voltage clamp recordings, especially tail current recordings, can you tell me some basic references.
25 August 2014 8,943 4 View
During organ bath, I am using aorta as a model, how I know that the function of endothelium detoriated beside using acetylcholine?
25 June 2014 6,300 8 View
Or, how and by which mechanism can hydrogen sulfide modulate endothelial dysfunction?
16 June 2014 1,832 3 View
01 May 2014 7,566 1 View
Is a shortening of the telomeres one of the limitations?
25 April 2014 1,494 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
