"What are the main challenges in optimizing CRISPR-Cas9 gene editing efficiency for therapeutic applications?"
In the qualitative compound report obtained from HR-LCMS analysis of crude plant extracts, what is meant by Hits (DB)? Should we consider all the predicted compounds from the list for further studies?
30 June 2024 2,290 4 View
In my LC-MS experiment, I am utilizing an Agilent Polaris NH2 column to separate a mixture of glucose, fructose, and sucrose. I am using water and acetonitrile as A and B mobile phases...
26 March 2024 8,895 4 View
Or is it used only to analyze the modelled protein structures.
02 March 2024 6,662 2 View
Not able to view the downloaded model from ClusPro using PDBSum. Since both the docked proteins contain single A chain, PDBSum shows it as single image. Please give suggestions. Also tried to save...
18 January 2024 5,286 4 View
I am using a C30 column for carotenoid analysis. I bought the column recently and using it for the first time. When I use the column in LC-MS scan mode, I clearly see the UV-Vis peaks for my...
08 June 2023 2,357 2 View
I am analyzing Lycopene using LC-MS. I use a C18 column and my mobile phase is Methanol: MTBE = 95:5. I use an Agilent Ultivo QQQ mass spectrometer with APCI negative ion mode. I see the precursor...
07 May 2023 525 0 View
I am running an HMBC experiment on a Bruker 300 mHz instrument. I set up the experiment following the steps given in TopSpin guide book. Everything happens fine but when I try to open the spectrum...
09 March 2023 5,289 4 View
I need to concentrate some fatty acid methyl esters in a cell culture medium to separate and isolate them using Prep-HPLC. What type of solid phase extraction would be suitable to concentrate...
02 March 2023 9,526 0 View
I used the following column for an HPLC separation; C18 4.6 x 250 mm, 5 um, 120 Ao. I used methanol and acetonitrile as mobile phases. Now I have to do LC-MS for the same samples. I am wondering...
19 December 2022 1,889 6 View
Hello, Can anyone please suggest the markers and protocol for FACS staining for macrophage from PBMC in human. Thank you.
09 March 2022 642 8 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
03 June 2024 2,877 7 View
Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...
30 May 2024 9,993 0 View