I used the following column for an HPLC separation; C18 4.6 x 250 mm, 5 um, 120 Ao. I used methanol and acetonitrile as mobile phases. Now I have to do LC-MS for the same samples. I am wondering whether I can use the same column. What factors should I consider and change? Thanks.
Yes you can. Just be carefull to the flow entering into the mass and adapt desolvatation temperatures and gases of the MS source to have a good spray.
Yes. depends on your method. If the method you use and the material you want to separate remains the same And able to provide good separation. As per Noel have mentioned, the mobile phase need to be volatile type.
Yes. In addition to another answers your need check compatibility of type your column by flow rate and column pressure for your MS ionization source. Also, You may need volatiles additives to solvents like formic acid for better ionization.
Yes, but if using any modifiers, make sure they are compatible with your LC-MS. If you are switching to LC-MS for not only specificity, but also sensitivity using something like formic acid will cause suppression, so you may want to modify. do your research!
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