PHA-P is the PHA protein before the further purification of PHA-E and -L. In the other hand, PHA-M is the mucoprotein form. It's said that these two kind of PHA could active T cells by different pathway. So, when I try to stimulate PBMCs to produce IL-2, IL-17, IFN-g, which PHA is better?
I have always used PHA-L at 1ug/ml to stimulate PBMCs . However I've been looking for differences between PHA-M and -P and as you said they are crude preparations although PHA-M has an associated polyscharide. If you already have PHA-M and -P and you want to try, I would suggest PHA-P since PHA-M is a weaker blood agglutinin than PHA-P, requiring 10-100 times more material to exhibit the same activity (see attachment PHA catalog from E-Y labs). However if you have to buy, I suggest PHA-L.
Good luck!
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