What markers are the best markers for hepatocellular carcinoma?

More Aiken Huang's questions See All

Are these tumours in the mouse small intestine?

We knock out a tumor suppressor gene in mouse to see whether it can promote the spontaneous development of intestine tumor. We were very happy as we observed some tumour-like lesions in the...

10 November 2016 390 9 View

What are the differences between PHA-P and PHA-M?

PHA-P is the PHA protein before the further purification of PHA-E and -L. In the other hand, PHA-M is the mucoprotein form. It's said that these two kind of PHA could active T cells by different...

01 February 2013 5,624 2 View

How do drugs get into the circulation by intraperitoneal injection?

Intraperitoneal injection (IP injection) is a common used method in animal study. I found that the pharmacokinetic of a drug given by IP was different to that by gavage. Apart from that the...

08 September 2012 1,495 22 View

Is thalidomide a real TNF-a inhibitor?

Maybe thalidomide is one of the most controversial drug in the medicinal history. It was initially withdrew from the market 'cause of its teratogenecity tragedy. Then it raised a lot of interest...

07 August 2012 7,046 4 View

Do Ca2+ and Mg2+ in HBSS decrease B16-F10 in vivo metastasis ability?

I have found that in most papers Ca2+ and Mg2+ free HBSS was used to prepare B16-F10 cells suspension for the in vivo metastasis assay. I performed a similar assay recently, but unfortunately used...

06 July 2012 8,086 8 View

Arthus reaction: Ovalbumin or bovine serum albumin (BSA), which antigen is better?

I would like to perform a reversed passive arthus reaction assay by intravenous injection of antigen and then intradermal injection of antibody into rats. Using BSA and anti-BSA antibody pair is...

06 July 2012 7,034 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How much should I centrifuge cells in agarose-coated plates to get spheroids?

Right now I am using HT29 cells, but I will also use HCT116 and probably breast cancer cell lines.

09 February 2021 2,063 6 View

C-4 I cervical cancer cell line tissue culture protocol?

Hi there! My lab has been brought an array of C-4 I cell line. The cells did not succeed in attaching to the bottom of the flask and remained floating in the media. Although we followed the...

04 February 2021 10,018 5 View

Aiken Huang

Thank you Jose.

However, no all HCC is GPC3 positive. What I need is a marker to showed that the sample determined by FCM is from HCC but not other tissue.

AFP is a useful marker, but it has the same problem as well, that no all HCC express AFP.

Yes, I found an antibody called anti human hepatocyte clone OCH1E5, sold by Dako company. It marks hepatocyte specially. However I am not sure if this antibody is suitable for FCM (not listed on the website). Does anyone have some suggestions?