Hi, I am new to this. I have done sequence alignment of modified tRNA genome sequences from E. coli and Mycobacterium tuberculosis against the whole genome database of tRNA and have not found any hit showing the similarity in their sequences. I am trying to do Mycobacterial translational complex studies and would like to know how relevant it is to reconstitute Mtb ribosome with E. coli tRNAs. My query protein binds to tRNA as well as mycobacterial ribosome in vivo. I am performing biochemical and structural studies.
I am not speaking from direct experience, but considering the lack of sequence similarity between M. tb. and E. coli tRNAs you mentioned, I would not want to use E. coli tRNA for the study. It is probably quite difficult to prepare tRNA pools from M. tb, however, so you may instead choose to use M. smegmatis. At least it's in the same genus, and it is easier and safer to work with.
Check this link maybe helpful
Article TRNA is a new target for cleavage by a MazF toxin
regards
https://www.nature.com › nature communications › articles
https://www.sciencedirect.com/science/article/.../S009286740080123...
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