I am planning to use blocking primer for MEMO-PCR method so I will put a blocker in the 3' end. I wonder if C3 spacer and phosphate have differences in efficiency or others.
In my mind i want to use heating coil but this coil cannot control the temperature itself. if anyone know what instrument that i can use to do this partitioning test at 60C
21 January 2024 4,254 1 View
I m making 10 percent seperating gel but my both gel stacking and resolving are not solidify plz let me know how to resolve this issue
01 January 2020 8,434 3 View
I did sequencing run using PhiX control in Illumina's NextSeq 500 and found out the %PF only 0.4% yet the cluster density reached 267K/mm2. The reagent I used was fresh (not expired yet), it was...
21 August 2017 6,198 3 View
I did COLD-PCR for JAK2 wildtype and mutant samples. The previous results were okay, the peaks were around 76-77 celcius. Now the peaks are around 77-78, I wonder what happened. My samples are...
03 March 2016 6,762 4 View
I am going to detect BRAF v600e mutation in colorectal cancer sample and currently looking for appropriate methods to compare and to use. I am thinking of doing MEMO-PCR but still lack of...
07 February 2016 3,737 1 View
I am looking for a free program that will search a known DNA sequence with known PCR primers, and find degenerate matches. If I put my DNA sequence and primers into NCBI Primer Blast, for...
31 August 2015 4,038 3 View
Can someone explain how to do an unpaired t test for the percentage amount for cd14 and cd16, which were stimulated with lps, for 0, 24h and 48h. This experiment was carried out 3 times. Do i...
13 August 2015 949 3 View
I placed a sample of my cells in a flow cytometer, and I saw a monocyte population, but I also saw another cell population that were smaller than monocytes but more granular than them also....
06 July 2015 3,514 2 View
I use acrylamide gel with 8.5% concentration with 50ml volume but I think it takes too long waiting them forming unto gel, about more than 8 hours, even overnight. In contrast, my friend also use...
24 June 2015 1,789 4 View
I use Geneaid KIT to use the gel extraction protocol because I need to purify my samples that have been amplified with v1-v3 primer and reamplified with v1-v3 primer GC clamp. The results...
03 May 2015 3,248 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View