8 Questions 2 Answers 0 Followers
Questions related from Juli Elisa Udayani
I did sequencing run using PhiX control in Illumina's NextSeq 500 and found out the %PF only 0.4% yet the cluster density reached 267K/mm2. The reagent I used was fresh (not expired yet), it was...
22 August 2017 6,059 3 View
I did COLD-PCR for JAK2 wildtype and mutant samples. The previous results were okay, the peaks were around 76-77 celcius. Now the peaks are around 77-78, I wonder what happened. My samples are...
04 March 2016 6,631 4 View
I am planning to use blocking primer for MEMO-PCR method so I will put a blocker in the 3' end. I wonder if C3 spacer and phosphate have differences in efficiency or others.
02 March 2016 6,363 0 View
I am going to detect BRAF v600e mutation in colorectal cancer sample and currently looking for appropriate methods to compare and to use. I am thinking of doing MEMO-PCR but still lack of...
08 February 2016 3,707 1 View
I use acrylamide gel with 8.5% concentration with 50ml volume but I think it takes too long waiting them forming unto gel, about more than 8 hours, even overnight. In contrast, my friend also use...
25 June 2015 1,629 4 View
I use Geneaid KIT to use the gel extraction protocol because I need to purify my samples that have been amplified with v1-v3 primer and reamplified with v1-v3 primer GC clamp. The results...
04 May 2015 3,110 2 View
My goal in my research is to analyse the dynamics of bacterial community structures in water samples from a zero-water discharge system. I might not sequence all the bands so I need to know if...
12 November 2014 1,691 1 View
It's been common to use primer for variable regions in 16s DNA but I heard that it's not highly trusted anymore. I wonder what primer is now used for DGGE and where I can get it.
08 November 2014 5,315 4 View
