I want to perform western In winter temperature below 25 degree centigrade My gel is not solidify what to do to resolve this issue?

I m making 10 percent seperating gel but my both gel stacking and resolving are not solidify plz let me know how to resolve this issue

01 January 2020 8,215 3 View

Why does my %PF on PhiX run in NextSeq 500 so low (0.4%)?

I did sequencing run using PhiX control in Illumina's NextSeq 500 and found out the %PF only 0.4% yet the cluster density reached 267K/mm2. The reagent I used was fresh (not expired yet), it was...

21 August 2017 5,965 3 View

Why my melting curve analysis results from same samples change in different PCR run?

I did COLD-PCR for JAK2 wildtype and mutant samples. The previous results were okay, the peaks were around 76-77 celcius. Now the peaks are around 77-78, I wonder what happened. My samples are...

03 March 2016 6,573 4 View

How do I find degenerate PCR primers?

31 August 2015 3,824 3 View

How long does it take to form 8.5% acrylamide gel with 50ml volume for DGGE?

I use acrylamide gel with 8.5% concentration with 50ml volume but I think it takes too long waiting them forming unto gel, about more than 8 hours, even overnight. In contrast, my friend also use...

24 June 2015 1,578 4 View

Why does my gel excision do not result in 800 ul after given 500 ul DF buffer and dissolved completely at 57 C for 15 minutes using Geneaid KIT?

I use Geneaid KIT to use the gel extraction protocol because I need to purify my samples that have been amplified with v1-v3 primer and reamplified with v1-v3 primer GC clamp. The results...

03 May 2015 3,062 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

Why my protein band in SDS-PAGE if heating the sample becomes much lighter compared to not heating the sample?

I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...

15 February 2021 3,080 3 View

Which power-to-heat (P2H) devices do you think are likely to play the most crucial role in the European energy transition?

Power-to-heat (P2H) shows how we can use renewables for heating. Power-to-heat technologies have been on the market for quite some time now, with heat pumps being the most important. However, they...

10 February 2021 9,250 2 View

I'm looking for HVAC relevant topic for thesis?

Heating Ventilation Air-conditioning System related thesis topic for thesis

09 February 2021 4,215 5 View

In Abaqus, is it possible to remove elements which are too complex for meshing from a sequence obtained by getByBoundingBox() ?

Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...

03 February 2021 8,198 1 View

Is my beginners approach to ansys fluent udf implementation realistic?

Hello dear online comunity, i am new to CFD and we are currently involved in a project work for our masters degree. Since the problem we decided to simulate is quite complex, we cannot rely on the...

29 January 2021 2,200 3 View

What is the average lifetime of ODLAB acid inert heating plates?

Dear Colleagues, common hotplates have a short lifetime when used for acid digestion of rock/mineral samples. PTFE/graphite coated hotplates are much more expensive and should live much more than...

21 January 2021 1,208 3 View

For what purposes is it advisable to use small solar linear-focus collectors (PTC, Fresnel reflectors)?

For example, the length of the collector is up to 10 m, the diameter is 1.5-2 m. It seems that flat collectors are usually used for individual solar heating and / or hot water supply.

13 January 2021 8,928 3 View