I use Geneaid KIT to use the gel extraction protocol because I need to purify my samples that have been amplified with v1-v3 primer and reamplified with v1-v3 primer GC clamp. The results contained primer dimers and smears a lot so I need to purify it before using it as samples in DGGE. When I did the protocol, the solution of DF buffer and my dissolved gel excision were less than 800 ul. I wonder what went wrong there.
Did you cut your band with a scalpel? Then the sharper is your gel excision the lesser will be the yield after heat-dissolution of agarose band. On the other hand, the thicker is the band the higher will be the yield of liquids after heat-dissolution. This is not really crucial, don't worry.
I do not think the volume after gel is dissolved is crucial here. You did nothing wrong. The volume depends upon both buffer and the size of gel slice. The smaller the gel pieces, the lesser the volume.
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